| |
Microbiological
parameters |
G |
I |
Minimum
sampling frequency |
Method
of analysis and inspection |
1 |
Total
coliforms/100 ml |
500 |
10 000 |
Fortnightly
(1) |
Fermentation
in multiple tubes. Subculturing of the positive tubes on a confirmation medium. Count
according to MPN (most probable number) or membrane filtration and culture on an
appropriate medium such as Tergitol lactose agar, endo-agar, 0.4% Teepol broth,
subculturing and identification of the suspect colonies. In the case of 1 and 2, the
incubation temperature is variable according to whether total or faecal coliforms are
being investigated. |
2 |
Faecal coliforms/100 ml |
100 |
2 000 |
Fortnightly
(1) |
3 |
Faecal streptococci/100 ml |
100 |
- |
(2) |
Litsky method. Count
according to MPN (most probable number) or filtration on membrane. Culture on an
appropriate medium. |
4 |
Salmonella/litre |
- |
0 |
(2) |
Concentration by membrane
filtration. Inoculation on a standard medium. Enrichment - subculturing on isolating agar
- identification |
5 |
Enteroviruses
PFU/10
litres |
- |
0 |
(2) |
Concentrating by
filtration flocculation or centrifuging and confirmation |
| |
| |
Physico-chemical
parameters |
G |
I |
Minimum
sampling frequency |
Method
of analysis and inspection |
6 |
pH |
- |
6-9 (0) |
(2) |
Electrometry with
calibration at pH 7 and 9. |
7 |
Colour |
- |
No
abnormal change in colour (0) |
Fortnightly
(1) (2) |
Visual inspection or
photometry with standards on the Pt.Co scale. |
8 |
Mineral oils mg/litre |
£ 0.3 |
No film
visible on the surface of the water and no odour |
Fortnightly
(1) (2) |
Visual and olfactory
inspection or extraction using an adequate volume and weighing the dry residue. |
9 |
Surface-active substances
reacting with methylene blue mg/l (Lauryl sulphate) |
£ 0.3 |
No
lasting foam |
Fortnightly
(1) (2) |
Visual inspection or
absorption spectro-photometry with methylene blue. |
10 |
Phenols mg/l (phenol
indices) C6 H5 OH |
£ 0.005 |
No
specific odour
£ 0.05 |
Fortnightly
(1) (2) |
Verification of the
absence of specific odour due to phenol or absorption spectro-photometry 4-aminoantipyrine
(4 A.A.P.) method. |
11 |
Transparency |
2 |
1 (0) |
Fortnightly
(1) |
Secchi's disc. |
12 |
Dissolved oxygen %
saturation O2 |
80 to
120 |
- |
(2) |
Winkler's method or
electrometric method (oxygen meter). |
13 |
Tarry residues and
floating materials such as wood, plastic articles, bottles, containers of glass, plastic,
rubber or any other substance. Waste or splinters |
Absence |
- |
Fortnightly
(1) |
Visual inspection. |
14 |
Ammonia mg/litre NH4 |
- |
- |
(3) |
Absorption
spectrophotometry, Nessler's method, or indophenol blue method. |
15 |
Nitrogen Kjeldahl mg/litre
N |
- |
- |
(3) |
Kjeldahl method. |
| |
| |
Other
substances regarded as indications of pollution |
G |
I |
Minimum
sampling frequency |
Method
of analysis and inspection |
16 |
Pesticides mg/litre
(parathion, HCH, dieldrin) |
- |
- |
(2) |
Extraction with
appropriate solvents and chromatographic determination. |
17 |
Heavy metals such as:
arsenic mg/litre As cadmium Cd chrome VICr VI leadPb mercury Hg |
- |
- |
(2) |
Atomic absorption possibly
preceded by extraction. |
18 |
Cyanides mg/litre Cn |
- |
- |
(2) |
Absorption
spectrophotometry using a specific reagent. |
19 |
Nitrates
mg/litre NO3 and
phosphates PO 4 |
- |
- |
(2) |
Absorption
spectrophotometry using a specific reagent . |